Ultraviolet Light / Transilluminators

Transilluminators are a source of potentially hazardous ultraviolet (UV) radiation. They are currently a prescribed electronic device under the Radiation Safety Act and must therefore be registered with the S&H  and Radiological Council.

A transilluminator uses an intense source of UV radiation to make visible the fluorescent marker used in electrophoresis gels. The gel is placed on the transilluminator window and is illuminated from below. Ultraviolet radiation is hazardous to both skin and eyes. Exposure to UV radiation at the surface of a transilluminator can exceed the NHMRC maximum permissible occupational exposure levels for skin and eyes in less than one second.

Transilluminators may be fitted with a UV absorbing cover. If the unit is operated without the cover in place or has no cover, then all exposed skin and the eyes of the operator must be protected. Protection is achieved by wearing a face shield which complies with Australian Standards 1336 & 1338.2 and wearing substantial clothing (such as long sleeves and laboratory coats) and heavy-duty gloves. Note that disposable vinyl gloves are not suitable, as they are transparent to UV radiation.

A number of transilluminator models are in common use. They produce UV light with varying intensities and spectra. It should be assumed that all are a UV hazard. If there is any doubt, the Safety and Health can provide the characteristics of each model.

UV Transillumunitor Working Rules